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Dexamethasone has variable effects on mesenchymal stromal

Dexamethasone (Dex) is a potent synthetic member of the glucocorticoid class of steroid drugs. Frequently, Dex has been used to enhance osteogenic, chondrogenic and adipogenic differentiation of mesenchymal stromal cells (MSC). Recently, Dex was applied to promote MSC proliferation, because of the rare frequency of MSC in bone marrow, and could. Interestingly, mice treated with Dex exhibited significantly prolonged survival (73 vs 21 days, p<0.01) and had a lower percentage of blasts (50%, p<0.01) and MSC compared to leukemic mice receiving vehicle treatment. Our data thus identified Dex mediated abrogation of AML-MSC interaction is in part mediated by affecting Notch signaling While Dex/AscP did not increase bone formation, they significantly stimulated BMSC in vitro proliferation without affecting the number of BMSC colonies formed by the colony-forming units-fibroblasts. We conclude that for the substantial majority of donors, Dex/AscP have no effect on the ability of BMSCs to form bone and myelosupportive stroma. DEX attenuate stroma expression. Over-proliferated fibroblasts, dense matrix collagen and hyperpermeable vessels are features of tumor stroma and are responsible for hindering delivery of anticancer drug or nanomedicines delivering into tumor tissue and into cells . Several markers have been used to detect cancer-associated fibroblasts (CAFs. There were few assayable osteoprogenitors in either the AP(high) or AP(low) fractions in the absence of dexamethasone (dex), suggesting that RBM stroma contains largely dex-dependent osteoprogenitor populations, and that dex may regulate osteoprogenitors subsequent to the upregulation of AP

AML Associated Mesenchymal Stroma Cells Support Growth of

  1. Differential effects of DEX on chondrogenesisDOI: 10.22203/eCM.v022a23 ISSN 1473-2262 Abstract Mesenchymal stromal cells (MSCs), which reside within various tissues, are utilized in the engineering of cartilage tissue. Dexamethasone (DEX) - a synthetic glucocorticoid - is almost invariably applied to potentiate the growth
  2. Dex was chosen as a key factor because of its effects on osteoblastic and adipocytic differentiation capabilities [12-23,26,27]. Interestingly, Dex effects were shown to be dependent on cell differentiation stage. Indeed, in cells committed to the osteogenic lineage, such as MC3T3-E1 and ROS 17/2.8 cells, Dex stimulated osteogenesis [28,29]
  3. However, combination therapy with MSCs and DEX (i.e., MSC+DEX) did not yield an increased survival benefit with no mouse surviving more than 24 h. On the other hand, treatment with DEX@M&Ms, at 1 mg kg −1 or an 80% reduced dose of DEX compared with free DEX, significantly increased survival with 100% survival observed at 120 h
  4. Herpes zoster (HZ), or shingles, results from reactivation of latent infection with varicella- zoster virus, which also causes chicken­pox. Anyone who has had chickenpox, even in subclinical form, is at risk for developing HZ. It is estimated that the lifetime risk of HZ is 30%, and 1 mil­lion new cases are reported annually in the United.

Marrow-derived stromal cells (MSCs) maintain the capability of self-renewal and differentiation into multiple lineages in adult life. Age-related changes are recognized by a decline in the stemness potential that result in reduced regeneration potential of the skeleton. To explore the molecular events that underline skeletal physiology during aging we catalogued the profile of gene expression. stromă. stromelor. ( bot.) grup de lamele între care se găsește clorofila unor plante. ( bot.) organ de rezistență al unor ciuperci, în care se află elementele de înmulțire. ( anat.) rețea de suport a unui organ din țesut conjunctiv, vase și nervi Human bone marrow stromal cells were examined for their osteogenic potential in an in vitro cell culture system. Dexamethasone (Dex) treatment induced morphological transformation of these cells from an elongated to a more cuboidal shape, increased their alkaline phosphatase activity and cAMP responses to PTH and prostaglandin E2, and was essential for mineralization of the extracellular matrix Summary The inhibitory effects of glucocorticoids (GCs) on bone marrow stromal stem cell (BMSC) proliferation and osteoblastic differentiation are an important pathway through which GCs decrease bone formation. We found that microRNA-34a-5p was a critical player in dexamethasone (Dex)-inhibited BMSC proliferation and osteogenic differentiation. MicroRNA-34a-5p might be used as a therapeutic.

In vivo formation of bone and haematopoietic territories

  1. B Significantly altered GR target genes of PF179TCAF-shGR-1 cells, after control DMSO treatment as well as after 100 nM Dex, 100 nM Dex + 1 µg/ml Dox, and 100 nM Dex and 12 µM RU486 treatment.
  2. Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2 Dev Biol . 1994 Jan;161(1):218-28. doi: 10.1006/dbio.1994.1022
  3. Stroma — may refer to: *Stroma, Scotland, an island off the northern coast of Scotland *Stroma (animal tissue), the connective, functionally supportive framework of a biological cell, tissue, or organ *Stroma, the thick fluid found in between grana, which Wikipedia. stromă — STRÓMĂ, strome, s.f. (bot.) 1. Grup de lamele între care.
  4. The GR dimer in stromal cells mediates the induction of genes of anti-inflammatory macrophages after DEX treatment in serum transfer-induced arthritis (STIA). (A) Quantitative RT-PCR of isolated ankle mRNA extracts showing the regulation of CD163, CD36, AnxA1, Axl and MerTK after 72 hours of DEX treatment in wt→wt and wt→GR dim mice
  5. These data suggested that thymic involution as a result of Dex or irradiation treatments induced similar alterations in the stromal composition including expansion of K5 + K8 + early TEC precursors. Fig. 6
  6. Quantitation of cell surface AP protein by flow cytometry indicated that culture with dex or BMP-2 produced a mixed population of cells with low AP (dim cells) and cells with high AP levels, while the combination of dex + BMP-2 yielded very few dim cells and a population of cells containing higher AP levels than with either inducer alone

Enhancing solid tumor therapy with sequential delivery of

Abstract. Studies examining the regulation of the type 3 deiodinase (D3) have been hampered by the lack of cell lines that constitutively express this enzyme. In vitro studies have shown that combination of scaffolds, bone marrow stromal cells and Dex-loaded CMCht/PAMAM dendrimer nanoparticles (3-D system) enhanced osteogenesis. Finally, in vivo studies have shown that the novel Dex-loaded CMCht/PAMAM dendrimer nanoparticles may be beneficial as intracellular nanocarrier, supplying Dex in a. Statin-Dex promoted the development of Foxp3 + Treg cells among thymocytes with the existence of thymic stromal cells in vitro. All these data demonstrated that statin-Dex may induce central tolerance in EAMG, which may provide a new strategy in the treatment of myasthenia gravis dexamethasone (Dex), ascorbic acid (Asc) and β-glycerophosphate (β-Gly). This review describes the effects of these substances on intracellular signaling cascades that lead to osteogenic differentiation of bone marrow stroma-derived stem cells. We conclude that Dex induces Runx2 expression by FHL2/ β-catenin-mediated transcriptional.

The total and adherent cultures were used to determine whether Dex and AscP directly affected the adherent stromal population or whether the effect was mediated through the nonadherent population. Cells were cultured in the presence or absence of Dex and AscP Interestingly, mice treated with Dex exhibited significantly prolonged survival (73 vs 21 days, p<0.01) and had a lower percentage of blasts (50%, p<0.01) and MSC compared to leukemic mice.

The aim of the present investigation was to compare the effects of cyclic compression, perfusion, dexamethasone (DEX) and bone morphogenetic protein‑7 (BMP‑7) on the proliferation and differentiation of human bone marrow stromal cells (hBMSCs) in polyurethane scaffolds in a perfusion bioreactor. Polyurethane scaffolds seeded with hBMSCs were cultured under six different conditions, as. Strategies to disrupt these deterministic obstacles require a unique combination of promoter drugs and cytotoxic agents to target stroma and tumor simultaneously. Here, we engineered a novel strategy by co-delivery dexamethasone (DEX) and docetaxel (DTX) in the 2-in-1 liposome, namely (DEX + DTX)-Lip, with sequential release property Dexamethasone (Dex), a potent synthetic member of the glucocorticoid (GC) class of steroid drugs, is widely used as an immune suppressor. However, chronic Dex therapy is associated with side.

The standard procedure for the osteogenic differentiation of multipotent stem cells is treatment of a confluent monolayer with a cocktail of dexamethasone (Dex), ascorbic acid (Asc) and β-glycerophosphate (β-Gly). This review describes the effects of these substances on intracellular signaling cascades that lead to osteogenic differentiation of bone marrow stroma-derived stem cells A mouse bone marrow stromal cell line, ST2, is known to support osteoclast formation from mouse spleen cells in the presence of 1,25(OH) 2 D 3 and dexamethasone (Dex) [41]. OPG bound to a single class of high-affinity binding sites induced by 1α,25(OH) 2 D 3 and Dex in ST2 cells [21]. When the binding sites on the treated ST2 cells were. Dex reduced aggrecanase activity in (RADA)4 and agarose hydrogels, as measured by anti-NITEGE Western blotting, for both bovine and human BMSC-seeded gels. Conclusions—The effects of Dex on matrix production are dependent on cell source and hydrogel identity. This is the first report of Dex reducing aggrecanase activity in a tissu Stromal cells are an important component of the bone marrow, and the extracellular matrix proteins secreted by these cells provide binding sites for hematopoietic stem and progenitor cells. 24-26 OP9 cells, derived from mouse bone marrow stroma, support the growth of hematopoietic stem and progenitor cells. 27 As shown in Figure 5A, there was a. Lineage commitment and differentiation of mesenchymal stromal cells (MSCs) into osteoblasts in vitro is enhanced by a potent synthetic form of glucocorticoid (GC), dexamethasone (Dex). Paradoxically, when used chronically in patients, GCs exert negative effects on bone, a phenomenon known as glucocorticoid-induced osteoporosis in clinical practice

Cell sorting enriches osteogenic populations in rat bone

Discussion: We demonstrated that pioglitazone and DEX can convert a stromal cell line, D1, from an osteoblastic phenotype that reversibly expresses adipocyte characteristics to terminally differentiated adipocytes. The antagonist of both nuclear receptors cannot inhibit the adipogenic effect of pioglitazone and DEX.Th Germ cell tumors and sex cord-stromal tumors are less common than epithelial tumors. The number of new cases a year in the US is 0.4 per 100,000 women and 0.2 per 100,000 women, respectively. In young people, sex-cord stromal tumors and germ cell tumors total 1% of overall ovarian cancer The supportive framework of an organ (or gland or other structure). The stroma is usually composed of connective tissue. It is distinct from the parenchyma, which consists of the key functional elements of that same organ (or gland or othe Mesenchymal Stromal Cells (MSCs) (Dex) over time. These microspheres were then encapsulated in a mixture of both hyaluronic acid (HA) and Pluronic F127, with the addition of MSCs. HA is a major component of the extracellular matrix (ECM) of articular cartilage and F127 is

Dex reduced aggrecanase activity in (RADA)[subscript 4] and agarose hydrogels, as measured by anti-NITEGE Western blotting, for both bovine and human BMSC-seeded gels. Conclusions: The effects of Dex on matrix production are dependent on cell source and hydrogel identity The synthetic glucocorticoid dexamethasone (dex) is commonly used in vitro in trilin-eage differentiation protocols for mesenchymal stromal cells (MSCs). In bone research, dex has been used for osteogenic differentiation of bone-marrow-derived MSCs (BMSCs) for almost 30 years [14], and differentiation cocktails containing at least 10 nM dex. Dex-induced differentiation of human bone marrow stromal cells was apparent after 2-3 days of treatment and reached a maximum at 7-14 days, as judged by alkaline phosphatase activity, although induction of osteocalcin by 1,25-dihydroxyvitamin D3 was attenuated by Dex Cuvantul stroma explicat in DEX. Dictio reprezinta peste 400000 de definitii, un dictionar explicativ extrem de usor de folosit ST2 cells, a cloned stromal-cell line from mouse bone marrow have the phenotypes of osteoblasts and hematopoietic supporting cells, but little is known about the adipogenesis in this cell line. We found that treatment of ST2 cells with a cocktail, a combination of insulin, dexamethasone (DEX) and 3-isobutyl-1-methylxanthine (IBMX), induced.

Stromal cells were grown as primary or first subcultures in the presence or absence of Dex and their expression of osteogenic markers (alkaline phosphatase activity, hormone responsiveness, and matrix molecules, including type I collagen, osteopontin, bone sialoprotein, and osteocalcin), as well as their functional capacity to differentiate to. Glucocorticoids, notably dexamethasone (Dex), have been reported to be a requirement for osteoprogenitor cell differentiation in young adult rat bone marrow stromal cell populations. We have reinvest..

MBA-15 cells were challenged with dexamethasone (Dex) or 17beta-estradiol and quantification of CReMM at the protein (ELISA) and mRNA (RT-PCR) levels had shown that Dex upregulated CReMM levels. Since CReMM is regulated by Dex, we analyzed the interaction of CReMM with the glucocorticoid receptor (GR), which mediates Dex action www.advancedsciencenews.com www.advancedscience.com factor,[8] TNF-stimulated gene 6,[9] and prostaglandin 2,[10] which endow MSCs the capabilities of suppressing proin-flammatory responses or switching proinflammation to anti The dense extracellular matrix (ECM) in tumor tissue severely hinders the penetration and enrichment of antitumor nanomedicines, which could significantly affect their efficiency. In this study, we used pH-sensitive nanocarriers loaded with collagenase (Col) to remold the tumor microenvironment (TME). Furth

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Dexamethasone in osteogenic medium strongly induces

Mesenchymal Stromal Cell‐Mediated Treatment of Local and

Dex-loaded CMCht/PAMAM dendrimer nanoparticles could play a crucial role in the regulation of osteogenesis, in vivo. Macroporous hydroxyapatite (HA) scaffolds were seeded with rat bone marrow stromal cells (RBMSCs), whose cells were expanded in MEM medium supplemented with 0.01 mg ml−1 Dex The aim of the present investigation was to compare the effects of cyclic compression, perfusion, dexamethasone (DEX) and bone morphogenetic protein‑7 (BMP‑7) on the proliferation and differentiation of human bone marrow stromal cells (hBMSCs) in polyurethane scaffolds in a perfusion bioreactor. Polyurethane scaffolds seeded with hBMSCs were cultured under six different conditions, as. The feasibility of Dex-BBA as a photoinitiator to initiate the gelation of aminoethylmethacylate-modified collagen (Coll-AEMA) was examined with or without the presence of stroma cells. Results. The Dex-BBA crosslinked hydrogels were weaker than the EDC crosslinked constructs

Herpes Zoster Ophthalmicus - American Academy of

Transcriptional profiling of mesenchymal stromal cells

Introduction. Bone marrow stroma is a complex tissue consisting of many cell types, which provide a microenvironment for haematopoiesis and also contribute to the maintenance and regeneration of skeletal tissues , , .Perturbed function of stromal tissue in humans can cause severe defects of skeletal system , , , or dysregulation of haematopoiesis resulting in myelodysplasia and acute myeloid. Research Hospitalization Volume, DRGs, Quality Outcomes, Top Hospitals & Physicians for C49A - Gastrointestinal stromal tumor - ICD 10 Diagnosis Cod

stromă - Wikționa

While Dex repressed genes related to adipogenesis and catabolism, this decrease was complementary to an increase in expression of genes related to osteogenesis. Conclusion: This study summarizes the genes expressed in the ex vivo cultured mesenchymal cells and their response to Dex Research Hospitalization Volume, DRGs, Quality Outcomes, Top Hospitals & Physicians for C49A1 - Gastrointestinal stromal tumor of esophagus - ICD 10 Diagnosis Cod

Differentiation of human bone marrow osteogenic stromal

Influence of biomechanical and biochemical stimulation on

Glucocorticoids impair bone formation of bone marrow

stroma. stroma. The supporting connective tissue of an organ or plastid. Glossary of Biotechnology for Food and Agriculture . 2015. stringent plasmid; structural gene; Look at other dictionaries Adipose stromal cells, harvested from fresh adipose specimens, were grown to confluence, switched to SF media, and then incubated in the presence or absence of DEX with CM from T47-D breast cancer cells, pre-treated with or without 17β-estradiol (E2), and with CM from stromal cell cultures with rat marrow stromal cells, treatment with FGF-2 and DEX decreases DNA content as compared to FGF-2 alone.21 In the present study, we developed an efficient method for the ex vivo expansion of ATSCs using FGF-2 in combination with steroids, and showed that ATSCs that are expanded in the presence of FGF-2 and DEX maintain their multi-lineag The Dex -sensitive, MM.1S (CRL -2974), and Dex-resistant, H929 (CRL -9068), MM cell lines and bone marrow derived mesenchymal stromal cells (BM-MSCs; PCS-500-012) were purchased from the American Type Culture Collection (ATCC). The cells were cultured in medium (HyClone; GE Healthcare Life Sciences) containing 10% feta Electronic Supplementary Information Activated macrophage-targeted dextran−methotrexate/folate conjugate prevents deterioration of collagen-induced arthritis in mice Modi Yang,a Jianxun Ding,*b Ying Zhang,b Fei Chang,*c Jincheng Wang,c Zhongli Gao,*a Xiuli Zhuangb and Xuesi Chenb aDepartment of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China

PPT - Selinexor Synergizes with Dexamethasone throughEffects of pharmacological and genetic disruption of CXCR4

In the interest of optimizing osteogenesis in in vitro, the present study sought to determine how porcine bone marrow stromal cell (BMSc) would respond to different concentrations of hyaluronan (HY) and its different combinations with dexamethasone (Dex) and recombinant human bone morphogenic protein-2 (rhBMP-2) Stromal Cells Dendritic Cells IL-6 TNF IL-1 A IL-2 IFN CD8+ T Cells C E Bone Marrow Vessels ICAM-1 VEGF bFGF D B NK Cells NK-T Cells Hideshima et al. Blood 96: 2943, 2000 Davies et al. Blood 98: 210, 2001 Gupta et al. Leukemia 15: 1950, 2001 Mitsiades et al. Blood 99: 4525, 2002 Lentzsch et al Cancer Res 62: 2300, 2002 LeBlanc R et al. Blood.

Suppression of autophagic activation in the mouse uterus

Response of stromal cells to treatment with 1,25(OH)2D3 and Dex RANKL, OPG, and M-CSF mRNA were expressed by cultured skin fibroblastic cells. RANKL mRNA expression was increased by 1,25(OH)2D3/Dex by 8 h compared with control, and this increase was sustained until the 24-h time point (Fig. 1A). OPG levels were, in contrast, greatly re Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesis in vitro . Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the aspect plastic adherence without any further. A mouse bone marrow-derived stromal cell line, ST2, is known to support OCL formation from mouse spleen cells in the presence of 1,25(OH) 2 D 3 and dexamethasone (Dex) . We have recently reported that OPG/OCIF specifically binds to ST2 cells treated with 1,25(OH) 2 D 3 and Dex, but not to untreated ST2 cells ( 8 , 9 )